Support Topics

How do I use LightField to do absorbance measurements? [+][-]

Q: How do I use LightField to do absorbance measurements?

A: On-line absorbance measurements are easy to perform within LightField using the following steps.

  1. Select “Regions of Interest” and then set up two Custom Regions of Interest that are equal in size.
  2. Under "Online Processes", check Apply Formula and then select Edit Formula

    online process
  3. Under “Pre-Defined Formulas”, select Absorbance.
    predefined formulas

  4. LightField will automatically select ROI 1 as the reference channel and ROI 2 as the transmitted channel. You can reverse these channels if necessary.
  5. The output will be Log10(reference/transmitted)

  6. Run or Acquire data

  7. For these measurements we suggest that you acquire a new background reference (Online Corrections) and also use a shutter during acquisition.


I need to reinstall my software but have lost the password. What do I do? [+][-]

Please submit a copy of the Sales Order to: 

A new key will be provided. If you do not have the sales order, please provide the serial number of the camera that was purchased with the software.

A formula that sets a threshold for an image and then multiplies this result by the orginal input (image intensity data). [+][-]


Clipping: Line 1: k = threshold (input, 1000) Line 2: output = input * k

This formula sets a threshold for an image and then multiplies this result by the orginal input (image intensity data). All the data above the threshold remain unchanged while data below the threshold is set to a value of zero (0).

Note: The formula example above includes a threhold of 1000 counts. You should change this value to correspond to the intensity dtaa in your specific experiment.

Original image:


Original image (top) vs. Image after LightField Math (bottom)

Note: Both images above were scaled to show background intensity. Peak intensities are equal for the original and modified images, while the background in the bottom image has been completely subracted.



Wavelength Calibration with Synchronized Data [+][-]

Before collecting any spectroscopic images or spectra using LightField, we recommend that you first perform an IntelliCal wavelength calibration.
Why should you perform a wavelength calibration when collecting an image, especially when the image only displays pixel position?


How do I normalize my data? [+][-]

Q: How do I normalize data?

A: To normalize intensity, use the following formula:

output=input/reduce(input, maximum)

This formula scales the data so the highest peak is normalized to one.

This can be applied during acquisition under Online Processes, or in post processing under Processes - Formula

normalize data

IMPORTANT: Do not use normalization during live acquisition when running step and glue because it will normalize every frame as acquired resulting in mismatches between the spectral data when it is stitched together.


Create a binary image using LightField Math [+][-]

Threshold: output = threshold(input, 975)

This formula basically creates a binary image. Intensities below the threshold level are converted to zero (0), while intensities above the threshold are converted to a value of one (1)

Notes: In this example, intensity data of 975 and above is converted to a value of one (1), while data with intensity below 975 is converted to zero (0).

You should change the threshold value of 975 to a number that corresponds to the desired threshold level in your specific data file.

Original Image:


Formula Results:


Calculate Average Pixel Intensity in Real Time [+][-]

Calculate Average Pixel Intensity in Real Time: output = reduce(input[53:293,47:186], average)

This formula courtesy of Dr. Sebastian Remi, PI’s Northeast Sales Engineer, displays the average pixel intensity of an ROI in real time during Run or Acquire. You can also select F6 to display statistics for the file or ROI. This will update during live acquisition, however only at a rate of approximately one update every 1-2 seconds.

Note: The numeric input “53:293,47:186” shows the ROI for the formula. Instead of typing in the numbers that define the ROI, you can use the input selection wizard in the formula editor. It looks like this:

When you select Run or Acquire, average intensity is displayed live however you will not see a live image.


Do you offer any drivers for LightField software? [+][-]

Q: Do you offer any drivers for LightField software?

A: Yes, we offer the following:

  • LightField Automation
  • SITK
  • DLL for LabVIEW

Contact your sales representative for additional information.

If LightField not displaying individual frames [+][-]

When averaging or summing multiple frames, LightField does not display individual frames as they are collected. Instead, LightField displays the “waiting for first live frame” message.
How can I see individual frames as they are collected?


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