Products: IsoPlane Imaging Spectrographs

image of IsoPlane Imaging Spectrographs

Best-in-Class Imaging Spectrometers

IsoPlane imaging spectrometers set new standards of performance and versatility.

The patented, multi-award–winning IsoPlane-320 is the best imaging spectrometer in its class. Fast f/4.6 optics and exceptional image quality ensure maximum throughput and signal-to-noise performance. A unique optical design completely eliminates astigmatism across the focal plane for superior multichannel capabilities. There is simply nothing like the IsoPlane-320.

The IsoPlane-160 is a compact 200 mm imaging spectrometer featuring fast f/3.88 optics, a unique astigmatism-corrected optical design, excellent imaging performance, and spectral resolution that rivals most 1/3 meter Czerny-Turner (CT) spectrometers. The best-in-class IsoPlane-160 offers all of this performance and versatility in a compact design.

IsoPlane features include:

  • High spectral resolution
  • Excellent imaging performance
  • Superior signal-to-noise ratio
  • Patented optical design (IsoPlane-320)
  • Triple-grating turrets with auto-turret and
    grating-identification functions


Princeton Instruments’ tradition of technical innovation continues with the multi-award–winning IsoPlane-320 spectrometer, whose exclusive zero-astigmatism optical design provides sharper images and spectra for dramatically improved resolution and signal-to-noise ratio. Resolution comparable to that of a ½ meter spectrometer, with twice the light-gathering power, makes the IsoPlane-320 the instrument of choice for demanding low-light-level applications.

The IsoPlane-160 offers the spectral resolution of a 1/3 meter spectrometer in a compact package. Its unique f/3.88 optical design virtually eliminates astigmatism while providing excellent spectral resolution, making it ideal both for spectroscopy and microspectroscopy applications.

Applications for the IsoPlane spectrometers include

  • Micro-spectroscopy: Raman, fluorescence and photoluminescence (PL)
  • Multichannel spectroscopy
  • Raman spectroscopy
  • Fluorescence and photoluminescence
  • Laser-induced breakdown spectroscopy (LIBS)
  • Biomedical imaging
  • Fourier-domain spectroscopy
nanoparticle applicationArray of chiral gold nanoparticles - Image courtesy of Saulius Juodkazis, Swinburne University of Technology, Melbourne, Australia.
IsoPlane 320 - Patented, astigmatism-free design
  • Zero astigmatism at all wavelengths across the entire focal plane gives high spatial resolution
  • Resolve >100 optical fiber channels with minimal crosstalk
  • Excellent results with CCD cameras with heights of up to 22 mm
  • No other mirror-based scanning spectrograph offers comparable performance.


High fluence
  • More photons per pixel increases spectral resolution and contrast
  • Greater signal intensity maximizes sensitivity  (C6H6 1460 cm-1 image).
Triple-grating turret under complete software control
  • Access any region from the UV to mid-IR with software-selectable center wavelengths for complete experimental flexibility, unlike lens based spectrographs
  • Optimize diffraction efficiency and resolution for any experiment with a single mouse click
  • Triple grating turrets can be changed in less than a minute, permitting access to up to 9 gratings.
Precision micrometer focus adjustment
  • Optimizes camera and spectrograph focus.
  • Provides razor-sharp images and spectra.
micrometer focus
High-efficiency optical coatings
  • Acton #1900 enhanced-aluminum mirrors provide highest reflectivity from 193 nm to mid-IR.
  • Optional  protected silver coatings provide an average > 98% reflectivity.
  • Optional gold coatings for NIR.


Compatible with wide variety of Princeton Instruments cameras
  • PIXIS CCD cameras
  • ProEM EMCCD cameras
  • PI-MAX4 ICCDs and emICCD cameras
  • NIRvana InGaAs NIR/SWIR cameras
Wide range of optional accessories
  • Fiber optic bundles, filter wheels, light sources, gratings and more.


Powerful LightField software
  • Optional: LightField® (for Windows® 7/8, 64-bit) or WinSpec (for Windows XP®/7/8, 32-bit).
  • Flexible software packages for data acquisition, display, and analysis.
  • LightField offers intuitive, cutting-edge user interface, IntelliCal, hardware time stamping, and more. Software sold separately.


IsoPlane Imaging Spectrograph model comparison and datasheets

Focal Length
Aperture Ratio
PMT Resolution*
CCD Resolution
320 mm
0.05 nm
0.08 nm
203 mm
0.10 nm
0.15 nm


Specifications determined with 1200 gr/mm grating at 435 nm with 26.8 mm width CCD camera with 20 μm pixels

*10 μm slit widths, 4 mm height

Filter by:  

Tip-Enhanced Raman Spectroscopy
TERS - Tip-Enhanced Raman spectroscopy

Laser-Induced Breakdown Spectroscopy
LIBS is considered one of the most convenient and efficient analytical techniques for trace elemental analysis in gases, solids, and liquids. LIBS spectra obtained by the Mars Curiosity Rover have confirmed that our sister planet could have harbored life

Fluorescence, Phosphorescence, and Photoluminescence Spectroscopy
Fluorescence, phosphorescence and photoluminescence occur when a sample is excited by absorbing photons and then emits them with a decay time that is characteristic of the sample environment.

General Raman
The most common application of Raman spectroscopy involves the vibrational energy levels of a molecule. Incident laser light in the UV, visible or NIR, is scattered from molecular vibrational modes.

Surface-Enhanced Raman Spectroscopy
SERS - Surface-enhanced Raman spectroscopy

Coherent Anti-Stokes Raman Spectroscopy
Coherent Anti-Stokes Raman spectroscopy (CARS) a type of non-linear Raman spectroscopy. Instead of the traditional single laser, two very strong collinear lasers irradiate a sample.

Singlet Oxygen Imaging
Singlet oxygen, the first excited state of molecular oxygen, is a highly reactive species that plays an important role in a wide range of biological processes, including cell signaling, immune response, macromolecule degradation, and elimination of neoplastic tissue during photodynamic therapy.

Combustion researchers rely on laser-based optical diagnostic techniques as essential tools in understanding and improving the combustion process.

Plasma Emission Spectroscopy
The different types of plasma emission spectroscopy can be categorized by how the plasma is generated. Spectra of nuclear fusion plasmas are used to ascertain the chemical species present and other properties.

Resonance Raman Spectroscopy
Instead of fluorescence, some types of colored molecules produce strong Raman scattering at certain conditions. This effect was called Resonance Raman.

Stimulated Raman Scattering
Stimulated Raman scattering takes place when an excess of Stokes photons that were previously generated by normal Raman scattering are present or are deliberately added to the excitation beam.


Zachary Schultz, et. al.
Probing Membrane Receptor - Ligand Specificity with Surface- and Tip- Enhanced Raman Scattering
Researchers at Northwestern University used an IsoPlane 320 and a ProEM EMCCD camera in their SERS setup to investigate interactions between a ligand and a protein.
Yong-Le Pan, et. al.
Detection and characterization of chemical aerosol using laser-trapping single-particle Raman spectroscopy
Researcher at the US Army Research Lab and Sandia National Lab utilize a IsoPlane 320 and a ProEM EMCCD to perform Raman spectroscopy to detect and characterize the presence of chemical agent aerosals in various complex atmospheric environments for a defense mission.
R. Pasqualotto, et. al.
Progress on Development of SPIDER Diagnostics
Within the path towards the development of ITER heating neutral beam injectors (HNB), a key role is played by SPIDER, the full size prototype of the HNB source under construction at Consorzio RFX [1]. SPIDER is a 100 kV negative hydrogen/deuterium RF source, with a multi-grid accelerator, made of 1280 apertures distributed over an area of 1.5x0.6m2, in 4x4 groups of 5x16 apertures each.
J. Zaumseil, Y. Zakharko et. al.
Multispectral electroluminescence enhancement of single-walled carbon nanodisk arrays
The integrations of periodic nanodisk arrays into the channel of a light-emitting field-effect transistor leads to enhanced and directional electroluminescence from thin films of purified semiconducting single-walled carbon nanotubes.
André Bardow, et. al.
A milliliter-scale setup for the efficient characterization of isothermal vapor-liquid equilibria using Raman spectroscopy
A research team from RWTH Aachen University in Germany used an IsoPlane 320 spectrograph with a ProEM 1600 EMCCD camera in their experimental setup measurement procedure for the rapid determination of isothermal vapor-liquid equilibria using only milliliter samples.
G. Borstad,J. Ciezak-Jenkins
Hydrogen-Bonding Modification in Biuret Under Pressure
An IsoPlane SCT320 spectrograph with an air-cooled PIXIS 400BR eXcelon CCD and the LightField software (Princeton Instruments) were used to collect the Raman spectra
A. Graf, J. Zaumseil et al.
Near-infrared exciton-polaritons in strongly coupled single-walled carbon nanotube microcavities
Research teams from Germany and the UK use Fourier Plane spectroscopy to show strong coupling of Carbon Nanotubes in microcavities. This research could lead to electrically pumped electron-polariton lasers.
C. Krafft, J. Popp et al.,
Raman-based Identification of Circulating Tumor Cells for Cancer Diagnosis
An IsoPlane 160 spectrograph and PIXIS camera were used by researchers in their presentation of the use Raman-based methodologies to distinguish cancer cells from normal blood cells. In a first approach, a microfluidic chip was developed to collect Raman spectra from optically trapped cells.
F. Diaz, S. Palomba et al.
Sensitive method for measuring third order nonlinearities in compact dielectric and hybrid plasmonic waveguides
Researchers at University of Sydney, UCal Berkeley and Lawrence Livermore Labs incorporate the IsoPlane and NIRvana to demonstrate a sensitive method for the nonlinear optical characterization of micrometer long waveguides, and apply it to typical silicon-on-insulator nanowires and to hybrid plasmonic waveguides.
D. Roxbury, D. Heller et al.
Cell Membrane Proteins Modulate the Carbon Nanotube Optical Bandgap via Surface Charge Accumulation
NIRvana 640 SWIR/NIR camera and IsoPlane 320 are integral parts of the experimental setup in cell adhesion research involving carbon nanotubes.
S. Cueff, R. Zia et al.
Dynamic control of light emission faster than the lifetime limit using VO2 phase-change
This proof-of-concept demonstration shows how integration with phase-change materials can transform widespread phosphorescent materials into high-speed optical sources that can be integrated in monolithic nanoscale devices for both free-space and on-chip communication.
D. Li, R. Zia et al.
Quantifying and controlling the magnetic dipole contribution to 1.5-µm light emission in erbium-doped yttrium oxide
NIRvana SWIR/NIR camera and IsoPlane 320 spectrograph are instrumenatal in recent groundbreaking research.

Application Notes

Aberration-Free Spectrographs and NIR-Sensitive InGaAs Cameras Facilitate the Development of Carbon Nanotube Optical Sensors for Early Disease Detection
Dr. Daniel Heller and his research group at Memorial Sloan Kettering Cancer center utilized the IsoPlane 320 spectrograph and NIRvana SWIR camera in their recent research.

Advanced CCD Cameras and Imaging Spectrographs Facilitate Acquisition of Novel Femtosecond Stimulated Raman Spectroscopy Data To Improve SERS Biosensors
Accurate characterization of surface-enhanced Raman spectroscopy (SERS) biosensors, fluorescent dye molecules that hold great promise for in vivo bioanalyte detection, can often be quite difficult as the overwhelming isoenergetic fluorescence signal typically makes it challenging to measure resonance Raman cross-sections for the molecules. To overcome this obstacle, researchers at the University of Minnesota in Minneapolis recently utilized etalonbased femtosecond stimulated Raman spectroscopy (FSRS), a technique designed to acquire a stimulated Raman signal without strong fluorescence or interference from signals resulting from other four-wave mixing pathway

Low-Frequency Raman Spectra of Amino Acids Measured with an Astigmatism-Free Schmidt-Czerny-Turner Spectrograph: Discovery of a Second Fingerprint Region
Low-Frequency Raman Spectra of Amino Acids Measured with an Astigmatism-Free Schmidt-Czerny-Turner Spectrograph: Discovery of a Second Fingerprint Region

Microscopy and Raman Imaging: Open-system Raman microscopy
Author: Cynthia Hanson and Elizabeth Vargis
05/05/2015  Publication: Laser Focus Wolrd
An IsoPlane 160 and a PIXIS 400 CCD camera are part of a cost effective Raman microscope solution developed at Utah State University. View the article in Laser Focus World.

Real-Time Imaging of Singlet Oxygen via Innovative Microspectroscopy Instrument
New Two-Dimensional InGaAs Detector Thermoelectrically Cooled to –85°C Facilitates Scientific Research

Scientific NIR-II/ SWIR Cameras Enable Femtosecond Frequency Comb Vernier Spectroscopy
New, Deeply Cooled InGaAs Cameras Provide Ultrahigh Sensitivity for Key Spectral Range

Acquiring and processing Raman spectral data for the C2-D stretching vibration of 2 deuterated histidine
Because of histidine’s importance and unique functionality, we wanted to map out the probe group’s sensitivity to allow for its general use in protein related research.

Solar cell inspection via photoluminescence imaging in the NIR/SWIR
Scientific-grade, deep-cooled, large-format InGaAs FPA cameras such as the NIRvana from Princeton Instruments will enable researchers to observe photoluminescence emission at longer wavelengths and rapidly obtain more detailed information about defects within multicrystalline silicon solar cells.

Ultra-High-Speed, Time-Resolved Spontaneous Raman Scattering Spectroscopy in Combustion
The recent use of a new diagnostic apparatus to measure the dynamics of each individual molecular species, as opposed to simply acquire bulk information (e.g., pressure), points to the possibility of performing temperature and frequency analyses of species in combustion.

Low-frequency Raman Spectra of Carbon Nanotubes Measured with an Astigmatism-free Schmidt-Czerny-Turner Spectrograph
An IsoPlane has been interfaced to a low frequency Raman module that enables measurement of peaks with Raman shifts as low as 10 cm-1 using only a single stage spectrograph.


Product Manuals
Download operation manuals for Princeton Instruments cameras, spectrometers, and accessories from our ftp site.


LightField 64-bit software - trial download
Download a 45-day free trial of Princeton Instruments' Revolutionary 64-bit data acquisition package for spectroscopy and imaging.

Tech Notes

IntelliCal-Automated wavelength and intensity calibration routines significantly improve accuracy of recorded spectra
Automated wavelength and intensity calibration routines significantly improve accuracy of recorded spectra.

Fully automated wavelength calibration method optimizes data accuracy
Currently offered as a LightField package option, patent-pending IntelliCal® technology from Princeton Instruments enables fast, reliable wavelength calibration with minimal user input.

Better Imaging with a Schmidt-Czerny-Turner Spectrograph
The IsoPlane spectrograph has a unique optical design that completely eliminates field astigmatism at all wavelengths and at all points across the focal plane, and reduces coma to negligible levels. This means the IsoPlane gives sharp and spatially well resolved images across the entire CCD sensor.

Improved Spectra with a Schmidt-Czerny-Turner Spectrograph
The data in this paper have shown that by decreasing optical aberrations and increasing fluence, the IsoPlane gives spectra with better spectral resolution and SNR compared to Czerny-Turner spectrographs. Higher spectral resolution means peaks that are too close together to be resolved by a CT spectrograph can be clearly seen with the IsoPlane.

Technical Posters

Aberration-Free Raman Spectroscopy with the IsoPlane
This poster shows that the well-known Raman spectra of carbon tetrachloride and cyclohexane are obtained with higher resolution and signal-to-background ratios with the IsoPlane spectrographs than with a conventional 300 mm C-T instrument.

Tips & Tricks

LightField Tips and Tricks
These tips and tricks focus on LightField features to enhance the user experience when operating Princeton Instruments spectroscopy systems.



ProEM EMCCD Cameras

ProEM EMCCD Cameras

EMCCD cameras for ultra-low light, read noise-limited applications.

NIRvana SWIR InGaAs Cameras

NIRvana SWIR InGaAs Cameras

Scientific grade, cooled InGaAs focal plane array cameras for demanding SWIR imaging and spectroscopy

PIXIS CCD Cameras for Imaging & Spectroscopy

PIXIS CCD Cameras for Imaging & Spectroscopy

PIXIS CCD cameras play a key role in revolutionary research performed in leading labs around the world.

LightField Scientific Imaging & Spectroscopy Software

LightField Scientific Imaging & Spectroscopy Software

Ground breaking software to control your Princeton Instruments systems. Now with Windows 10 support. It's like nothing you have ever experienced!



The reference standard of ICCD cameras. Single photon sensitivity and ultra-fast, <500psec gating

PyLoN Cameras for Imaging & Spectroscopy

PyLoN Cameras for Imaging & Spectroscopy

PyLoN high-resolution front-illuminated, back-illuminated, & back-illuminated deep-depletion imaging & spectroscopy CCDs.

PyLoN-IR Linear InGaAs Cameras

PyLoN-IR Linear InGaAs Cameras

This InGaAs detector offers 16-bit digitization and leads the industry with the fastest spectral rate and lowest system read noise.

Light Sources

Light Sources

Princeton Instruments offeres a variety of light sources for use with their spectrometers.

Fiber Bundles and Adapters

Fiber Bundles and Adapters

Several different fiber optic bundles are designed specifically for use with Princeton Instruments monochromators and spectrographs.

Filter Assemblies

Filter Assemblies

Our filter wheel assemblies are designed to hold up to six (6) 1.0” (25.4mm) diameter samples or filters.

Single Channel Detectors

Single Channel Detectors

A wide variety of single channel detectors for use with IsoPlane, SpectraPro and TriVista systems.

Grating and Turret Accessories

Grating and Turret Accessories

There are significant advantages of multi-grating turrets in spectrometers.



Princeton Instruments monochromators and spectrographs use diffraction gratings as the optical element which separates (disperses) polychromatic “white” light into individual wavelengths (colors).

IntelliCal Spectral Calibration System

IntelliCal Spectral Calibration System

Fully automated, spectral calibration system - for up to 10x more wavelength accuracy and instrument independent results

Princeton Instruments