Small Animal ImagingEducational Notes

Small Animal Imaging

X-ray and UV-VIS-NIR detection has been used for various scientific, military and medical applications. For medical imaging, X-rays provide good sample penetration, but are unable to give soft tissue information. UV and visible wavelengths are unable to penetrate soft tissue at all due to reflection and scattering of light.

Near infrared can be separated into two distinct windows. NIR-I encompasses wavelengths in the range 750 – 1060 nm, with NIR-II encompassing wavelengths 1060 – 1700 nm. NIR-I can be imaged via silicon CCDs but only offers a few millimeters of tissue penetration. NIR-II is able to penetrate deeper but can only be detected by InGaAs cameras.

Therefore, NIR-II methods have recently been developed for small animal imaging to investigate predominately early disease detection. Majority utilize fluorophores such as single-walled carbon nanotubes and quantum dots that fluoresce in the NIR-II region.

Application Notes

Deeply Cooled, Scientific InGaAs Cameras Facilitate NIR-II / SWIR Imaging for Drug Discovery / Small-Animal Research

For decades, x-ray and UV-vis-NIR detection methods have been used in various scientific, military, and medical applications. Although generally employed to good success, these systems nonetheless have some limitations when utilized for such types of work. Effectively leveraging the good sample penetration afforded by x-rays, for example, can prove difficult for…Read Full Article